Cytek Aurora 5L - Spectral Analyzer - Irchel 1

The Cytek Aurora is a 5 laser spectral flow cytometer with 64 fluorescence + 3 scatter channels that can process up to 30'000 events/s.

Spectral unmixing with the 64 fluorescence channels enables panels with up to about 60 colors. Cellular autofluorescence can be extracted during the unmixing process.

The installed, spatially separated lasers are: 355 nm (UV), 405 nm (Vio), 488 nm (Blue), 561 nm (YG) and 640 nm (Red).

Samples can be loaded using standard FACS tubes or 96 well plates.

Differences between Aurora 1 and 2:

Aurora 1 and 2 have the same optics and detection channels and differ only by the sample loader options.

• Aurora 1 has a plate loader that can cool and heat the samples in the plate but can only load standard 96 well plates.
• The loader on Aurora 2 has no temperature control but can load standard 96 well plates, 96 deep well plates and tube racks. We experienced less carry-over between samples when using the Aurora 2 compared to Aurora 1.

The SpectroFlo software can be used independently from the instrument on one of our virtual machines (FCF - Instrument S/W VM), e.g. for changing your unmixing or setting up your experiments.

See Data processing virtual machines for how to book and connect to the virtual machines.

Note that the software on the instrument is completely independent of the one on the virtual machine. All data/settings etc. have to be transferred via export/import. IMPORTANT: If you are using reference controls measured on different days you will need to import the QC data (PDF, 82 KB)!

The instrument is located on the Irchel Campus in 44 G 3f.

Biosafety note: All our analyzers run on a biosafety 2 permit. Please treat the workspace accordingly and indicate the biosafety level of your experiment in the instrument booking system.

If there is any doubt about the biosafety classification of your experiments please contact us  info@cytometry.uzh.ch

Usage of the instrument requires an introduction that is provided by the facility.

New users ►  please register with the facility.

Registered users ► please fill the web form on our online education platform (you will require your core account credentials for login). If you have further questions about the machine don't hesitate to contact us by email info@cytometry.uzh.ch