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The BD FACSymphony offers high-parameter single cell acquisition at high speed. Currently, the system is capable of recording 30 parameters (28 fluorescence channels + light scatter channels) at up to 40,000 events per second, and has the potential to be upgraded to 50 parameters.
With high power lasers and sensitive detectors the system offers improved sensitivity and resolution. Our FACSymphony is equipped with 5 spatially separated laser lines at 355 nm, 405 nm, 488 nm, 561 nm and 639 nm. The user has full control over the individual laser powers to further optimize acquisition conditions.
The high number of acquisition parameters and the high resolution make the FACSymphony the ideal instrument for the identification and analysis of rare cell types and in-depth or broad phenotyping of single cell events at high-throughput.
A High Throughput Sampler (HTS) for 96-well plates can be used for efficient high-throughput screening approaches but requires an additional introduction before usage.
Important: Please filter all your samples prior to measurement ( ⇒ Falcon #352235) and perform all washing steps described in the SOP to avoid clogging of the system.
Samples can only be loaded in 5 ml FACS tubes made of polystyrene (clear plastic e.g. FAL352058), polypropylene tubes (milky plastic like e.g. falcons or Eppis) will not pressurize. Minimum sample volume is 200 ul.
If you have sample volumes < 200 ul to load you can place an additional micronic tube in the 5 ml tube (e.g. Vitaris, Product no.: 32022-MIC).
The instrument is located on the Irchel Campus in 44 G 3f.
Biosafety note: All our analyzers run on a biosafety 2 permit. Please treat the workspace accordingly and indicate the biosafety level of your experiment in the instrument booking system.
If there is any doubt about the biosafety classification of your experiments please contact us info@cytometry.uzh.ch
Usage of the instrument requires an introduction that is provided by the facility staff.
New users ► please register with the facility.
Registered users ► please fill the web form on our online education platform (you will require your core account credentials for login). If you have further questions about the machine don't hesitate to contact us by email info@cytometry.uzh.ch
