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The NanoAnalyzer is a dedicated flow cytometer built specifically for analyzing nano-sized particles. Based on light scattering and fluorescence it can detect small biological particles down to ~40 nm in size.
The machine is equipped with a 488nm and 640 nm laser and records SSC and up to two fluorescent channels with highly sensitive photon avalanche diodes.
| Instrument specifications | |
|---|---|
| Optimal sample concentration | 1 x 108 - 1 x 109 particles/ml |
| Sample flow rate | 5 - 60 nl/min |
| Sheath flow rate | 10 - 40 µl/min |
| Calibrated sample size range for SSC |
S16M-Exo: 40 - 200 nm S17M-MV: 150 - 1000 nm |
| Maximum particle size to be loaded | 1 µm |
| Sample loading | 0.6 ml microtubes |
| Optimal sample event rate | 2000 - 12000 events/min |
Note: The samples can be exclusively loaded in 0.6 ml microtubes (MCT-060-L-C, Axygen or alternative supplier) which are not provided by the facility!
Compensation cannot be applied in the Nanoanalyzer software but data can be exported as FCS files to calculate compensation in different software.
The instrument is located at Y44G03 in the hallway.
Biosafety note: All our analyzers run on a biosafety 2 permit. Please treat the workspace accordingly and indicate the biosafety level of your experiment in the instrument booking system.
If there is any doubt about the biosafety classification of your experiments please contact us info@cytometry.uzh.ch
Usage of the instrument requires an introduction that is provided by the facility.
New users ► please register with the facility.
Registered users ► please fill the web form on our online education platform (you will require your core account credentials for login). If you have further questions about the machine don't hesitate to contact us by email info@cytometry.uzh.ch
