BD FACSAria III 5L - Lengg

4 lasers, 405 nm (violet),  488 nm (blue), 561 nm (yellow/green) and 633 nm (red) are spatially separated for excitation of 14 parameters. The 405 nm can be switched for a 375 nm near-UV laser with 2 channels as described in the optical configuration. 

70 µm, 85 µm, 100 µm and 130 µm nozzles are available for cell sorting of material ranging from bacteria to large cells like fat cells or tubules.

The sorter is operated by independent power users, power user in training and via operator based sorts.

Operator based sorts & training are available on request Mo - Fr from 09:00 - 17:00. Independet power user  can use the sorter 24/7.

Please consult our guidelines for sample preparation if you are unclear about how to prepare your cells for the sorting.

Samples as well as collected cells can be kept at desired temperature (4° - 42°C).

Sorted cells can be collected in following containers

• 4 x 5 ml tubes,
• 2 x 15 ml falcons,
• 4 x 1.5 ml eppis,
• 6 well, 24 well, 48 well, 96 well and 384 well plates
• onto microscopic slides or custom made collection devices

An aerosol management system allows cell sorting up to biosafety level 2 on this machine.

Contact info@cytometry.uzh.ch for further questions.

Address:  Forschungsgebäude Kinderspital, August-Forelstrasse 51, Floor D Room 4.020