BD FACSAria III 5L - Irchel 1

5 lasers, 405 nm (violet), 445 nm or 488nm (blue), 561 nm (yellow/green) and 633 nm (red) are spatially separated for excitation of up to 16 parameters as described in the optical layout.

70 µm, 85 µm, 100 µm and 130 µm nozzles are available for cell sorting of material ranging from bacteria to large cells like fat cells or tubules.

The sorter is operated by independent power users, power user in training and via operator based sorts.

Operator based sorts & training are available on request Mo - Fr from 09:00 - 17:00. Independet power user can use the sorter 24/7.

Please consult our guidelines for sample preparation if you are unclear about how to prepare your cells for the sorting.

Samples as well as collected cells can be kept at desired temperature (4° - 42°C).

Sorted cells can be collected in following containers

• 4 x 5 ml tubes,
• 2 x 15 ml falcons,
• 4 x 1.5 ml eppis,
• 6 well, 24 well, 48 well, 96 well and 384 well plates
• onto microscopic slides or custom made collection devices

We additionally offer custom made sample holders with following specifications:

• 4 x 2 ml eppis
• 1 x 5 ml tube and 3 x 1.5/2 ml eppis
• 1 x 15 ml falcon and 3 x 5 ml tubes
• 1 x 50 ml falcon and 2 x 5 ml tubes

An aerosol management system allows cell sorting up to biosafety level 2 on this machine.

Contact info@cytometry.uzh.ch for further questions.

Address: University Zurich, Winterthurerstrasse 190, Room Y44G03g, 8057 Zürich