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Cytometry Facility

BD FACSAria III 4L - Schlieren

Sorter Specifications

The following lasers are spatially separated available for excitation: 405 nm (violet), 488 nm (blue), 561 nm (yellow/green) and 635 nm (red). The instrument can record up to 15 parameters in parallel as described in the optical configuration.

70 µm, 85 µm, 100 µm and 130 µm nozzles are available for cell sorting of material ranging from bacteria to large cells like fat cells or tubules.

The sorter is operated by independent power users, power user in training and via operator based sorts.

Operator based sorts & training are available on request Mo - Fr from 09:00 - 17:00. Independet power user can use the sorter 24/7.

Please consult our guidelines for sample preparation if you are unclear about how to prepare your cells for the sorting.

Samples as well as collected cells can be kept at desired temperature (4° - 42°C).

Sorted cells can be collected in following containers

  • 4 x 5 ml tubes,
  • 2 x 15 ml falcons,
  • 4 x 1.5 ml eppis,
  • 6 well, 24 well, 48 well, 96 well and 384 well plates
  • onto microscopic slides

 

An aerosol management system allows cell sorting up to biosafety level 2 on this machine.

The sorter is located in Wagistrasse 12, floor K (fifth floor) room 104.

Contact info@cytometry.uzh.ch for further questions.

Telephone next to the sorter: 044 63 53 490

Address: Universität Zürich, Wagistrasse 12, Room WAD-K-104, Schlieren